Benefits of digital drip PCR:
- flexibility of the system and its optimization for EvaGreen and TaqMan probes of any manufacturer
- supersensitivity and superprecision of the quantitative analysis of the amplification products of the target gene in each drop of the sample
- fractionation of samples into nanodroplets minimizes the influence on the research result of such parameters as the presence of PCR inhibitors and efficiency
- the amplification proceeding due to the absolute counting of the target gene in each drop
- no need to use calibration curves; direct detection of a rare variant of a target gene in a complex environment
- Quantification of cancer biomarkers. Mutations associated with oncology often escape detection due to their low concentration compared to background wild-type DNA in the same sample. Due to its high sensitivity, the QX200 system allows detecting and quantifying mutations in concentrations from 0.0001% (!) - one in one hundred thousand.
- Detection and assessment of pathogens. Accurately quantifying viral load is essential for determining disease status and developing, validating, and evaluating therapy. QX200 has a sensitivity sufficient for detecting ultra-small amounts of viral genetic material, including in complex mixtures.
- Gene copy number determination (CNV). Gene copy number variation (CNV) is a significant source of polymorphism in the human genome. CNV has been associated with cancer, neurological and autoimmune diseases, and drug-related side effects. QX200 allows you to determine CNV with a ratio accuracy of 1.2x (!), For example, five against six.
- Validation and quantification of NGS libraries. With the help of digital PCR technology, it is possible to qualitatively and quantitatively evaluate the library, which allows the most efficient use of sequencers.
- Gene expression analysis. Drip digital PCR can quantify the level of gene expression. Due to the high accuracy and sensitivity of the method, it is possible to estimate a minimum 10% (!) Change in expression, including at low concentrations.
- Testing products for GMOs. With an accuracy of thousandths of a percent, it allows you to estimate the amount of genetically modified ingredients in food products, feed raw materials.