Sorbent Profinity eXact

Article BioRad 156300*

Order a product

Ask a question

More detailed

Delivery

We will bring, unpack, assemble

Convenient payment method

Leasing and deferred payment

Need a consultation?

Our specialists will answer any question you may have

Ask a question

Description
Specifications

Description

The Profinity eXact sorbent is part of a system for the expression, isolation and purification of recombinant proteins. Consists of a subtilisin protease conjugated to an agarose matrix. The main advantage of the system is that the subtilisin protease not only binds and recognizes the tag, but also quickly and accurately cleaves the tag from the recombinant protein to release the purified target protein.

Due to the specificity of recognition of the FKAL subtilisin tag, the purity of the eluted protein is usually higher than for other systems with affinity tags. The sorbent can be used in native and, if desired, denaturing conditions up to 4 M urea.

Benefits:

Cleaning and processing in one step;
Column digestion in as little as 30 minutes;
No protease addition required;
Exact cleavage at the N-terminus to obtain the target protein;
Stable over a wide pH range (2-13).

Features:

Method - liquid chromatography
Matrix - agarose
Functional group - modified subtilisin protease, 27.8 kDa
Recommended flow rate 1000 cm / h at 25 ° C
Capacity - ≥ 3 mg / ml (MBP, 40 kDa)
Particle size - 60-160 microns
pH stability - 2-13

Specifications

Country

USA

Back to the list