Sorbent CHT Ceramic Hydroxyapatite XT

Article BioRad 1200245*

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Description
Specifications

Description

CHT Ceramic Hydroxyapatite XT Media (CHT XT) is an affinity cation exchange sorbent that provides unparalleled separation and resolution properties. It can be used to purify enzymes, monoclonal and polyclonal antibodies of various classes, antibody fragments and bispecific antibodies; efficient isolation and purification of viruses and virus-like particles; and separation of supercoiled DNA from linear duplexes, as well as single-stranded from double-stranded DNA. Ideal for large scale biological processes.

Benefits:

Unique selectivity
Effective one-stage cleaning of aggregates and other impurities
High physical and chemical stability
Simple column packing

Features:

Method - liquid chromatography
Functional group - Ca²⁺, PO₄³¯, OH¯
Matrix - ceramic hydroxyapatite (Ca₁₀ (PO₄) ₆ (OH) ₂)
Maximum pressure - 150 psi (10 bar)
Dynamic capacity - 17-25 mg lysozyme / g sorbent; > 60 mg / ml IgG at a flow rate of 100 cm / h
Particle size - 40 microns
Recommended flow rate - 50-1000 cm / hour
Chemical stability - 1 N NaOH, 6 M urea, 8 M guanidine-HCl, ethanol, methanol, 100% acetonitrile
Autoclaving conditions - 121 ° C, 20 min in phosphate buffer, pH 7
pH stability: pH 6.5-14

Specifications

Country

USA

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