Block execution provides flexibility in solving various analytical problems; the ability to work in a semi-preparative and preparative mode using columns with an internal diameter of up to 20–50 mm when pumping a flow up to 150 ml / min; high degree of automation, the ability to remotely control via a local Ethernet network; Software in Russian.
Solvent supply systems with pumps, mobile phase flows from 0.1 μl / min to 10 ml / min:
- LC-20AT - with sequential double plunger pump;
- LC-20AD - parallel double plunger;
- LC-20AB - with two LC-20AD twin parallel plunger pumps, has a minimum dead volume;
- LC-20AP — with a double parallel plunger pump and an optional automatic plunger flushing system, it can operate in 2 modes:
- analytical - from 100 μl / min to 100 ml / min at a pressure of up to 42 MPa;
- perparative - from 100 ml / min to 150 ml / min at a pressure of up to 30 MPa.
Liquid degassing systems with a chamber volume of 380 ml:
- DGU-20A3 - has 3 degassing lines;
- DGU-20A5 - has 5 degassing lines.
Autosamplers SIL 20A (without refrigeration) and SIL-20AC (with refrigeration from 4 to 40 ° C):
- sample volume from 0.1 to 100 μl or from 1 μl to 2 ml (optional);
- increment of the specified sample volume, μl - 0.1 in the range from 0.1 to 0.9 μl or 1.0 in the range from 1 μl to 20 ml;
- the ability to operate autosamplers with various utensils:
- glass bottles and test tubes volume: 0.3; one; 1.1; 1.5; 4 ml;
- plastic test tubes with a volume of 0.2; one; 4 ml;
- 96 and 384 well plates;
- deep well 96-well plates;
- number of samples that can be analyzed in automatic mode:
- up to 50 in 4 ml tubes;
- up to 105 in 1.5 ml tubes;
- up to 175 in 1 ml tubes;
- up to 192 (2 x 96) in conventional or deep well 96-well plates;
- up to 768 (2 x 384) in conventional 384 well plates;
- up to 1152 (12 x 96) in conventional or deep well 96-well plates in combination with the RackChanger automatic plate changer;
- the number of injections per sample - from 1 to 30;
- reproducibility of injections - less than 0.3% (at 10 μl injections);
- sampling rate, μl / s - from 0.1 to 15 with an installation step of 0.1;
- injection accuracy - ± 1% (at 50 μl injections, 10x repetition);
- mixing a sample with 3 reagents in an autosampler makes it possible to:
- carry out a pre-column chemical reaction;
- automatically enter the internal standard;
- range of working pH values - from 1.0 to 14.0;
- maximum pressure, MPa - 20 (standard), 35 (option);
- SIL-HTA / HTC (no refrigeration / with refrigeration in the range of 4 - 40 ° C): sample volume - from 0.1 to 2 ml, up to 4 conventional or deep-well 96- or 384-well plates, up to 350 1 ml tubes, 140-210 tubes of 1.5 ml, up to 100 tubes of 4 ml, pressure - up to 20 MPa;
- SIL-10AF / AP: sample volume - from 1 to 50 μl / from 1 μl to 5 ml - standard; from 1 to 400 μl or from 1 μl to 2 ml - optional; increment step - 1 μl; the number of samples is from 25 to 192; the number of injections per sample is from 1 to 30.
Column thermostats automatically maintain a programmable temperature regime during chromatographic separation; a dosing valve and taps for column switching can be installed, allowing automatic column change:
- CTO-20A, from 10 ° C above room temperature to 85 ° C;
- CTO-20AC, cooled, from 10 ° C below room temperature to +85 ° C.
Detectors for LC-20 Prominence:
- spectrophotometric two-wave scanning detectors, path length - 10 mm, sample volume 12 μl, pressure up to 12 MPa, from 4 to 35 ° С:
- UV / VIS detector SPD-20A, from 190 to 700 nm;
- UV / VIS / NIR detector SPD-20AV, from 190 to 900 nm;
- diode array UV / VIS detector SPD-M 20A, path length 10 mm, volume 10 μl, spectra library, pressure up to 12 MPa, 4 to 35 ° С; from 190 to 800 nm;
- refractometric detector RID-10A for the detection of oligomers and polymers;
- spectrofluorometric detector RF-10AXL;
- conductometric detector CDD-10Avp.