Fermenter vessel description:
- borosilicate glass or stainless steel (customized)
- round bottom
- total / working volume - 2.0 l / 1.2 l; 3.6 l / 2.4 l; 7.5 l / 5.0 l and 13 l / 10 l
- dimensions of the vessel (the ratio of the depth of the vessel to the inner diameter):
- for a vessel 2 / 1.2 l - 240 x 115 (2.1: 1)
- for a 3.6 / 2.4 l vessel - 235 x 150 (1.6: 1)
- for a vessel of 7.5 / 5.0 l - 465 x 150 (3.1: 1)
- for a vessel 10 / 7.0 l - 352 x 200 (1.75: 1)
- for a 13/10 l vessel - 420 x 200 (2.1: 1)
- tight connection of the fermenter lid to the vessel without lubrication
- supplying the vessel and lid with handles for easy carrying
- temperature range - from 5 °C - 10 above the cooling water up to 60, optionally up to 80 (with a water jacket) and up to 90 (heating with a thermal cuff)
- vessels with direct heating - direct heating with a silicone thermo-cuff around the vessel, "cold finger"
- jacketed vessels - water heating by a heating element and water circulation provided by a circulation pump
- water cooling through a coil, cold water supply for cooling is controlled by an automatic valve
- 3 pumps with electronic control of dosing speed, 2 analog pumps
- automatic supply of reagents
- maximum pump speed - 17.9 ml / min
- the maximum number of built-in pumps - 5 pcs.
- the ability to connect external pumps
- stirrer rotation speed
- temperature
- pH
- dissolved oxygen concentration (option)
- foam level control (option)
- control of the mass flow of gases
- gas mixing system - GasMix (option)
- control of switching on and off the oxygen supply valve
- analysis of exhaust gases (O2, CO2)
- feeding with continuous cultivation
- optical density
- redox potential
- weight
- pressure
- analysis of the substrate (glucose and methanol levels)
Sterilization: Simultaneous autoclaving of the vessel along with the reagent and sampling bottles, pump heads, sensors, lines and air filters.
Features:
- control panel - color touchscreen
- independent control of 4 bioreactors from one control panel
- installation on a fermenter or remotely up to 15 m
- for touch panel:
- 1 GB memory allows you to save and view graphs without connecting to software
- shock protection, dust protection and moisture protection
- RS485, Ethernet, XML OPC DA
- for keypad:
- memory card for storing settings and calibration data
- RS232
Mixing process:
- stirring speed - 20–300 rpm
- removable electromagnetic stirrer motor located on top
- lack of bumpers on the vessel body
- mixing elements "sea propeller"
- Cellift, basket and other options on request
- special bubbler with a divider for creating small bubbles
- control of pH-medium for cell cultures, carried out using CO2
- gas mixing system - air, O2 and CO2 included
- the presence of a side filter (option) for the selection of the culture supernatant
Iris software allows you to:
- display fermentation parameters and display them in the form of a graph in one window
- write data to the computer memory, as well as transfer the recorded data to other programs
- set alarms for critical parameter values
- program the sequence of changing parameters during fermentation
- flue gas cooler
- pH-electrode, pO2-electrode, foam level sensor
- mass flow control, two-gas mixing system - air + O2, three-gas mixing system (air / O2 / N2), gas mixing system 3 + 1 (air / O2 / N2 + CO2)
- liquid level control sensor (for continuous fermentation)
- Iris V.5 software
- additional analog pump for power supply
- perfusion system with centrifugal filter
- various mixing elements and bubblers
- O2 and CO2 gas analyzer
- sampling system, multisampler
- immobilization systems for adherent cells
- cooling thermostat
- other accessories on request